KMT2D Knockout cell line (MS751)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

KMT2D Knockout cell line (MS751) Order

Catalog#

YKO-H766

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

KMT2D Knockout cell line (MS751)

Morphology

Epithelial-like, adherent

Passage ratio

1:2-1:3

Species

Human

Gene Name

KMT2D

Gene ID

8085

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate

Target region

Knockout region: Exon 4 (based on transcript KMT2D-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:MS751			STR Info (Cell bank) Cell Line:MS751
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	12	12		12	12
D7S820	9	11		9	11
D16S539	11	11		11	11
VWA	16	16		16	16
TH01	6	6		6	6
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	11	12		11	11

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KMT2D gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MS751 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KMT2D gene knockout KMT2D Knockout cell line (MS751) will be expanded and cryopreserved.

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KMT2D Knockout cell line (MS751)

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KMT2D Knockout cell line (MS751) Order

Detailed Product Information

Instruction

Related Popular Products

Wild-type Cell Lines

Human renal clear cell adenocarcinoma cells(MS751) More details Order

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit More details Order

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium More details Order

KO Cell Lines

Kmt2d Knockout cell line(ID8) inquiry More details

KMT2D Knockout cell line (HCT 116) inquiry More details

KMT2D Knockout cell line (HEK293) inquiry More details

Related Popular Products

Wild-type Cell Lines

Human renal clear cell adenocarcinoma cells(MS751)

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

KO Cell Lines

Kmt2d Knockout cell line(ID8)

KMT2D Knockout cell line (HCT 116)

KMT2D Knockout cell line (HEK293)

Gene Editing Services

EZ-editor™ Products

Contact us✖

Human renal clear cell adenocarcinoma cells(MS751)
More details Order

High-performance Mouse Genotyping Kit
More details Order

EZ-cryo™ Cell Freezing Medium
More details Order

Kmt2d Knockout cell line(ID8)
inquiry More details

KMT2D Knockout cell line (HCT 116)
inquiry More details

KMT2D Knockout cell line (HEK293)
inquiry More details